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Added ANI table
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_optionals/03-extra_tools.md

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@@ -24,6 +24,8 @@ The source code for **PyANI** is available at
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The documentation for basic usage is available
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[here](https://github.com/widdowquinn/pyani/blob/master/README_v_0_2_x.md){: target="_blank"}.
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The first stage of PyANI is
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**PyANI** v2 is available in Hipergator, but has to be loaded.
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The dependencies, `mummer` and `blast+` will be loaded together with `pyani`.
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@@ -83,8 +85,28 @@ ANIm_alignment_lengths.tab ANIm_percentage_identity.tab
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You can now transfer `ANIm_percentage_identity.png`
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to your computer to view the heatmap.
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For numeric values, you can use `ANIm_percentage_identity.tab` table.
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<img src="/fig/ANIm_percentage_identity.png" height="500px">
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Based on ANI, the unknown strain seems to be *X. hortorum pv. gardneri*.
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You can also get numeric ANI values from the `ANIm_percentage_identity.tab` file.
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~~~
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$ awk 'NR==1{print "-"$0; next}{for (i=2; i<=NF; i++) {$i=substr($i,1,5)}; print $0}' ani8/ANIm_percentage_identity.tab | column -t
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~~~
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{: .language-bash}
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~~~
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- Xpopuli Xhgardneri Xhcynarae Xhhederae Xhvitians Xhunknown Xhcarotae Xhtaraxaci Xhpelargonii
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Xpopuli 1.0 0.913 0.913 0.912 0.912 0.913 0.913 0.912 0.913
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Xhgardneri 0.913 1.0 0.993 0.960 0.982 0.999 0.961 0.974 0.958
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Xhcynarae 0.913 0.993 1.0 0.960 0.984 0.993 0.961 0.975 0.958
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Xhhederae 0.912 0.960 0.960 1.0 0.960 0.960 0.965 0.955 0.961
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Xhvitians 0.912 0.982 0.984 0.960 1.0 0.982 0.961 0.976 0.958
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Xhunknown 0.913 0.999 0.993 0.960 0.982 1.0 0.961 0.974 0.958
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Xhcarotae 0.913 0.961 0.961 0.965 0.961 0.961 1.0 0.956 0.961
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Xhtaraxaci 0.912 0.974 0.975 0.955 0.976 0.974 0.956 1.0 0.954
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Xhpelargonii 0.913 0.958 0.958 0.961 0.958 0.958 0.961 0.954 1.0
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~~~
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{: .output}
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Based on ANI, the unknown strain seems closest to *X. hortorum pv. gardneri*.

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