diff --git a/src/bfio/backends.py b/src/bfio/backends.py
index 9c7b227..ae7094b 100644
--- a/src/bfio/backends.py
+++ b/src/bfio/backends.py
@@ -1661,6 +1661,35 @@ def __init__(self, frontend):
 
             self._axes_list = []
 
+        def _get_axis_info(self):
+            shape_len = len(self._rdr.shape)
+            if shape_len == 5:
+                self._axes_list = ["t", "c", "z", "y", "x"]
+            else:
+                try:
+                    # OME-NGFF 0.5: multiscales is under attrs["ome"]["multiscales"]
+                    ome_meta = self._root.attrs["ome"]
+                    axes_metadata = ome_meta["multiscales"][0]["axes"]
+                    for axes in axes_metadata:
+                        self._axes_list.append(axes["name"])
+                except (AttributeError, KeyError):
+                    # Fall back to v2 location (attrs["multiscales"])
+                    try:
+                        axes_metadata = self._root.attrs["multiscales"][0]["axes"]
+                        for axes in axes_metadata:
+                            self._axes_list.append(axes["name"])
+                    except (AttributeError, KeyError):
+                        self.logger.warning(
+                            "Unable to find multiscales metadata. Z, C and T "
+                            + "dimensions might be incorrect."
+                        )
+                        if shape_len == 4:
+                            self._axes_list = ["c", "z", "y", "x"]
+                        elif shape_len == 3:
+                            self._axes_list = ["z", "y", "x"]
+                        elif shape_len == 2:
+                            self._axes_list = ["y", "x"]
+
         def read_metadata(self):
             self.logger.debug("read_metadata(): Reading metadata (v3)...")
             # First try OME metadata (same as v2)
@@ -1739,14 +1768,23 @@ def _init_writer(self):
                 with open(metadata_path, "w") as fw:
                     fw.write(str(self.frontend._metadata.to_xml()))
 
-                self._root.attrs["multiscales"] = [
-                    {
-                        "version": "0.1",
-                        "name": self.frontend._file_path.name,
-                        "datasets": [{"path": "0"}],
-                        "metadata": {"method": "mean"},
-                    }
-                ]
+                self._root.attrs["ome"] = {
+                    "version": "0.5",
+                    "multiscales": [
+                        {
+                            "name": self.frontend._file_path.name,
+                            "axes": [
+                                {"name": "t", "type": "time"},
+                                {"name": "c", "type": "channel"},
+                                {"name": "z", "type": "space"},
+                                {"name": "y", "type": "space"},
+                                {"name": "x", "type": "space"},
+                            ],
+                            "datasets": [{"path": "0"}],
+                            "metadata": {"method": "mean"},
+                        }
+                    ],
+                }
 
             # Check for existing arrays when appending
             if self.frontend.append is True:
diff --git a/tests/test_zarr_v3.py b/tests/test_zarr_v3.py
index 7a6fc70..18a4f66 100644
--- a/tests/test_zarr_v3.py
+++ b/tests/test_zarr_v3.py
@@ -1,5 +1,6 @@
 # -*- coding: utf-8 -*-
 """Tests for zarr v3 support in bfio using unittest."""
+
 import json
 import tempfile
 import unittest
@@ -97,6 +98,28 @@ def test_write_v3_ome_metadata(self):
             metadata_path = out_path / "OME" / "METADATA.ome.xml"
             self.assertTrue(metadata_path.exists())
 
+    def test_write_v3_multiscales_under_ome_key(self):
+        """Verify v3 writer stores multiscales under 'ome' key per NGFF 0.5."""
+        from bfio import BioWriter
+
+        with tempfile.TemporaryDirectory() as tmp:
+            out_path = Path(tmp) / "output.zarr"
+            data = numpy.random.randint(0, 255, (128, 128), dtype=numpy.uint8)
+
+            bw = BioWriter(out_path, backend="zarr3", X=128, Y=128, dtype=numpy.uint8)
+            bw[:128, :128, 0, 0, 0] = data
+            bw.close()
+
+            with open(out_path / "zarr.json") as f:
+                meta = json.load(f)
+            attrs = meta.get("attributes", {})
+            self.assertIn("ome", attrs)
+            self.assertIn("multiscales", attrs["ome"])
+            self.assertEqual(attrs["ome"]["version"], "0.5")
+            axes = attrs["ome"]["multiscales"][0]["axes"]
+            axis_names = [a["name"] for a in axes]
+            self.assertEqual(axis_names, ["t", "c", "z", "y", "x"])
+
 
 class TestZarr3Reader(unittest.TestCase):
     """Test Zarr3Reader reads v3 format stores."""
@@ -147,6 +170,40 @@ def test_read_v3_dimensions(self):
             self.assertEqual(br.T, 1)
             br.close()
 
+    def test_read_v3_axis_info_from_ome_key(self):
+        """Verify Zarr3Reader reads axis info from ome.multiscales per NGFF 0.5."""
+        from bfio import BioReader, BioWriter
+
+        with tempfile.TemporaryDirectory() as tmp:
+            out_path = Path(tmp) / "axis_test.zarr"
+            bw = BioWriter(
+                out_path,
+                backend="zarr3",
+                X=64,
+                Y=64,
+                Z=2,
+                C=3,
+                T=1,
+                dtype=numpy.uint8,
+            )
+            data = numpy.zeros((64, 64, 2, 3, 1), dtype=numpy.uint8)
+            bw.write(data)
+            bw.close()
+
+            # Verify the zarr.json has ome.multiscales with axes
+            with open(out_path / "zarr.json") as f:
+                meta = json.load(f)
+            axes = meta["attributes"]["ome"]["multiscales"][0]["axes"]
+            axis_names = [a["name"] for a in axes]
+            self.assertEqual(axis_names, ["t", "c", "z", "y", "x"])
+
+            # Verify reader correctly parses dimensions from ome key
+            br = BioReader(out_path, backend="zarr3")
+            self.assertEqual(br.Z, 2)
+            self.assertEqual(br.C, 3)
+            self.assertEqual(br.T, 1)
+            br.close()
+
 
 class TestZarrAutoBackendSelection(unittest.TestCase):
     """Test auto-detection picks correct backend."""