diff --git a/settings/.env.dev b/settings/.env.dev
index 3fd7bc1..49071b3 100644
--- a/settings/.env.dev
+++ b/settings/.env.dev
@@ -37,3 +37,9 @@ SEQREPO_ROOT_DIR=/usr/local/share/seqrepo/2024-12-20
 ####################################################################################################
 
 ENSEMBL_API_URL=https://rest.ensembl.org
+
+####################################################################################################
+# Environment variables for cdot
+####################################################################################################
+
+CDOT_URL=http://cdot-rest:8000
diff --git a/src/dcd_mapping/align.py b/src/dcd_mapping/align.py
index 18325d5..1d25546 100644
--- a/src/dcd_mapping/align.py
+++ b/src/dcd_mapping/align.py
@@ -21,7 +21,7 @@
 )
 from dcd_mapping.lookup import get_chromosome_identifier, get_gene_location
 from dcd_mapping.mavedb_data import LOCAL_STORE_PATH, patch_target_sequence_type
-from dcd_mapping.resource_utils import http_download
+from dcd_mapping.resource_utils import CDOT_URL, http_download
 from dcd_mapping.schemas import (
     AlignmentResult,
     GeneLocation,
@@ -377,7 +377,7 @@ def fetch_alignment(
         if accession_id.startswith(("NP", "ENSP", "NC_")):
             alignment_results[accession_id] = None
         else:
-            url = f"https://cdot.cc/transcript/{accession_id}"
+            url = f"{CDOT_URL}/transcript/{accession_id}"
             r = requests.get(url, timeout=30)
 
             try:
diff --git a/src/dcd_mapping/lookup.py b/src/dcd_mapping/lookup.py
index 017135e..08cf75b 100644
--- a/src/dcd_mapping/lookup.py
+++ b/src/dcd_mapping/lookup.py
@@ -51,7 +51,7 @@
 from gene.schemas import MatchType, SourceName
 
 from dcd_mapping.exceptions import DataLookupError
-from dcd_mapping.resource_utils import ENSEMBL_API_URL, request_with_backoff
+from dcd_mapping.resource_utils import CDOT_URL, ENSEMBL_API_URL, request_with_backoff
 from dcd_mapping.schemas import (
     GeneLocation,
     ManeDescription,
@@ -90,7 +90,7 @@ def seqfetcher() -> ChainedSeqFetcher:
 
 
 def cdot_rest() -> RESTDataProvider:
-    return RESTDataProvider(seqfetcher=seqfetcher())
+    return RESTDataProvider(url=CDOT_URL, seqfetcher=seqfetcher())
 
 
 # ---------------------------------- Global ---------------------------------- #
diff --git a/src/dcd_mapping/resource_utils.py b/src/dcd_mapping/resource_utils.py
index 01a67cd..6b610cf 100644
--- a/src/dcd_mapping/resource_utils.py
+++ b/src/dcd_mapping/resource_utils.py
@@ -10,6 +10,7 @@
 MAVEDB_API_KEY = os.environ.get("MAVEDB_API_KEY")
 MAVEDB_BASE_URL = os.environ.get("MAVEDB_BASE_URL")
 ENSEMBL_API_URL = os.environ.get("ENSEMBL_API_URL", "https://rest.ensembl.org")  # TODO
+CDOT_URL = os.environ.get("CDOT_URL", "cdot-rest:8000")
 
 LOCAL_STORE_PATH = Path(
     os.environ.get(
diff --git a/src/dcd_mapping/vrs_map.py b/src/dcd_mapping/vrs_map.py
index 18642ff..7bf3d15 100644
--- a/src/dcd_mapping/vrs_map.py
+++ b/src/dcd_mapping/vrs_map.py
@@ -31,6 +31,7 @@
     get_seqrepo,
     translate_hgvs_to_vrs,
 )
+from dcd_mapping.resource_utils import CDOT_URL
 from dcd_mapping.schemas import (
     AlignmentResult,
     MappedScore,
@@ -771,7 +772,7 @@ def store_accession(
 ) -> None:
     namespace = infer_namespace(accession_id)
     alias_dict_list = [{"namespace": namespace, "alias": accession_id}]
-    cd = cdot_rest()
+    cd = cdot_rest(url=CDOT_URL)
     sequence = cd.get_seq(accession_id)
     sr = get_seqrepo()
     sr.sr.store(sequence, alias_dict_list)