Impossible to open/read FAST5 files #5
Description
Hi there,
I am very interested in this software but I have difficulty to use my data.
I recive this error that tells me that is impossbile to open/read the FAST5 files.
I have checked if the path for the FAST5 files is correct and it is.
I am analysing barcoded data, one hypothesis is that I am using one f5.f5index that contains all the run but the BAM was about only 200 reads on 9Million sequenced.
Any suggestions ?
Error! cannot read fast5 file= /home/xxx/xxx/xxx/fast5/FAT20294_cfb18e17_1125.fast5
For: Qry= cb7ddec0-7da2-44e9-87c8-fc9bcfbe1930:717(717)-1518 with=1728/1728 Map= +chr4:3074876-3075648 Map-flag=0 qual= cigar-len=139/139
c_rep_regions_in_whole_0 chr4:3074876-3074940:3
c_rep_regions_in_whole_1 chr4:3074938-3074968:3
c_rep_regions_in_whole_2 chr4:3075017-3075049:12
c_rep_regions_0 chr4:3074876-3074933:3
Read fast5 file = /home/xxx/xxx/xxx/fast5/FAT20294_cfb18e17_1125.fast5
Error!!! Canot open file /home/xxx/xxx/xxx/fast5/FAT20294_cfb18e17_1125.fast5
HDF5-DIAG: Error detected in HDF5 (1.10.1) thread 140551523776320:
#000: H5G.c line 472 in H5Gopen2(): unable to open group
major: Symbol table
minor: Can't open object
#001: H5Gint.c line 287 in H5G__open_name(): group not found
major: Symbol table
minor: Object not found
#002: H5Gloc.c line 428 in H5G_loc_find(): can't find object
major: Symbol table
minor: Object not found
#003: H5Gtraverse.c line 867 in H5G_traverse(): internal path traversal failed
major: Symbol table
minor: Object not found
#004: H5Gtraverse.c line 639 in H5G_traverse_real(): traversal operator failed
major: Symbol table
minor: Callback failed
#005: H5Gloc.c line 383 in H5G_loc_find_cb(): object 'channel_id' doesn't exist
major: Symbol table
minor: Object not found