diff --git a/pyiceberg/catalog/rest/__init__.py b/pyiceberg/catalog/rest/__init__.py
index 3b77fd47f0..dfe8af009a 100644
--- a/pyiceberg/catalog/rest/__init__.py
+++ b/pyiceberg/catalog/rest/__init__.py
@@ -422,7 +422,12 @@ def add_headers(self, request: PreparedRequest, **kwargs: Any) -> None:  # pylin
                 params = dict(parse.parse_qsl(query))
 
                 # remove the connection header as it will be updated after signing
-                del request.headers["connection"]
+                if "connection" in request.headers:
+                    del request.headers["connection"]
+                # For empty bodies, explicitly set the content hash header to the SHA256 of an empty string
+                body = request.body
+                if body in (None, b"", ""):
+                    request.headers["x-amz-content-sha256"] = "e3b0c44298fc1c149afbf4c8996fb92427ae41e4649b934ca495991b7852b855"
 
                 aws_request = AWSRequest(
                     method=request.method, url=url, params=params, data=request.body, headers=dict(request.headers)