Use MetaFusion for hg38 based RNA reads

[Chih-Fan]

Hi,

I am trying to create reference files for MetaFusion to run on my fusion calling output on hg38 based RNA reads.
Can you provide me information about how the "new_bed.total.Oct-1-2020.uniq.bed" and "hgTables.gene_symbol.ENSG.ENST.ENSP.Nov-13-2020.tsv" files were made? Which regions are included here? Did you use a GTF file to create these references?

Thanks a lot.

Chih-Fan

[mapostolides]

Hi Chih-Fan,

At the moment, MetaFusion has annotations for hg19. However, if you use a tool like liftOver to convert the fusion caller coordinates from hg38 to hg19 coordinates, then you can use Metafusion as-is.

Best,
Michael