-Quality metrics for tracking and division detection. A-B. Cell Tracking Challenge format quality estimations in Table S3 are based on an ROI of a dataset (A), where each cell is manually tracked for as long it appears in the ROI. Manual track annotations are formatted in CTC format, to which automated tracking with TrackMate is then compared to estimate tracking quality. Division metrics in C-J are based on a dataset where each cell division is manually annotated (B). In this dataset, selected tracks are also annotated and compared to automated tracking in Table S3 and panel K. C-E, I. Division detections for Oneat (not connected with tracks), TrackMate-Oneat (Oneat divisions connected with TrackMate tracks), TrackMate-Oneat + MARI principle (TrackMate-Oneat with max boundary set for angle between mother cell and daughter cells), and TrackMate “native” track splitting, enabled in TrackMate LAP linking algorithm. F-H, J. Corresponding detection metrics. K. Manually annotated ground truth tracks colorized by the Track ID assigned by automatic tracking used for the experiments.
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