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Running the pipeline


Expected jobs

> snakemake --cores 2 -n

...
BAM_to_BED                                   2
Non_insertion_mapping                        2
all                                          1
annotation_overlap_insertion                 2
basic_insertion_plots                        1
build_insertion_reference                    1
calc_distance_to_elements                    2
calculate_exact_insertion_coordinates        2
clean_postcut_by_maping_quality              2
collect_outputs                              2
copy_config_version                          1
detailed_fragmentation_length_plot           2
extract_by_length                            2
extract_fastq_insertions                     2
extract_mapping_quality                      2
fasta_insertion_reads                        2
finalize_mapping_quality                     2
find_insertion_BLASTn                        2
find_insertion_BLASTn_in_Ref                 2
fragmentation_distribution_plots             2
generate_mapq_plot                           2
get_coordinates_for_fasta                    2
hardcode_blast_header                        2
insertion_fragmentation                      1
insertion_mapping                            2
insertion_points                             2
insertion_reads                              2
make_blastn_DB                               1
make_fasta_without_tags                      2
minimap_index                                1
multiqc                                      1
nanoplot                                     2
prepare_insertion                            1
read_level_fastqc                            2
sort_insertion_file                          2
split_fasta                                  2
total                                       63


All of these jobs will be executed in the correct order by the workflow. So let's finally run it.


Execution

    snakemake --cores 2 --use-conda

!!! info

Depending on the number of cores and threads specified and whether the environments need to be built for the first time, this process may take a while. However, since the simulated data is very small, the expected runtime should not exceed 5-10 minutes.

If you see this message, the workflow has executed successfully and completed. If not, refer to the error handling section.

    Finished job 0.
    63 of 63 steps (100%) done

Now, run snakemake again with its built-in --report functionality to get a comprehensive overview of the workflow's runtime and output.

    snakemake --report tutorial/report.html