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1. NOAA-PMEL-OME_eDNA_DiscFilters_Extraction_Protocol_Centrifuge_BeBOP NOAA-PMEL-OME_eDNA_DiscFilters_Extraction_Protocol_Centrifuge_BeBOP Public

   Nucleic acids extraction from 47 mm disc filters using the Qiagen DNeasy Blood and Tissue Kit with some modifications to the manufacturer’s protocol. These extraction protocols, adapted from Spens …

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2. NOAA-PMEL-OME_MetaMag_nad2_v1_qPCR_Protocol_BeBOP NOAA-PMEL-OME_MetaMag_nad2_v1_qPCR_Protocol_BeBOP Public

   This qPCR protocol is for amplifying and quantifying the nad2 gene in Dungeness crab (Metacarcinus magister).

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3. NOAA-PMEL-OME-Gel-Electrophoresis-Protocol-BeBOP NOAA-PMEL-OME-Gel-Electrophoresis-Protocol-BeBOP Public

   This protocol is for running and imaging PCR product using gel electrophoresis.

4. NOAA-PMEL-OME-Qubit-Quantification-Protocol-BeBOP NOAA-PMEL-OME-Qubit-Quantification-Protocol-BeBOP Public

   This protocol is for quantifying the concentration of DNA in the product of a DNA extraction.

5. NOAA-PMEL-OME_Universal_Sterilization_Protocol NOAA-PMEL-OME_Universal_Sterilization_Protocol Public

   Sterilization procedures used by the NOAA PMEL Ocean Molecular Ecology (OME) Group

6. NOAA-PMEL-OME_eDNA_Collection_Protocol_SmithRoot_PeristalticPump_BeBOP NOAA-PMEL-OME_eDNA_Collection_Protocol_SmithRoot_PeristalticPump_BeBOP Public

   Water collection and filtration protocol using Smith-Root self preservng eDNA filters to collect environmental DNA from marine ecosystems. This collection and filtration protocol is used by the NOA…