Custom scripts related to PacBio IsoSeq analysis
| Script | Input | Output | Requirements | Description |
|---|---|---|---|---|
| DemultiplexFlncFasta.py | 'isoseq_flnc.fasta' | Folder of barcode fasta files | BioPython, and tqdm python packages | Divides the pooled full-length reads from the classify step into individual fasta files based on barcode/primer. Requires that you added the custom primer/barcode file during the classify step. If done correctly, sequence descriptions should include the primer field*. |
>m151120_074440_42136_c100836382550000001823182312311555_s1_p0/98678/5437_73_CCS strand=-;fiveseen=1;polyAseen=1;threeseen=1;fiveend=25;polyAend=5389;threeend=5415;primer=0;chimera=0